The Seminal Fluid Isoenzyme LDH-C

609 Abstract: In order to determine the seminal activity of LDH-C4 and to investigate its correlation with other fertility indexes, we studed total LDH, total CK avtivities, and LDH-C4 in relation to sperm count, motility and sperm motility index (SMI). SMI is the measurement of electro-optical fluctuations caused by motile sperm. Total LDH and total CK were determined with a Technicon RA 1000 analyser, and LDH isoenzymes with Helena Lab. isoenzyme kits. The results in 23 infertile men showed that LDH-C4 activity was either absent (4 cases), or decreased (19 cases). LDH-C4 showed a positive correlation with total LDH, sperm count and SMI (p<0.01 r=0.5529, p<0.05 r=0.5025 and p<0.05 r=0.475, respectively). In addition, there were significant correlations between % C4 and total LDH activity, SMI,motility and sperm count (p<0.01 r=0.5529, p<0.05 r=0.475, p<0.05 r=0.503, and p<0.01 r=0.528, respectively). On the other hand, LDH-C4 activity/sperm count showed no correlation with any other index (sperm count, motility and SMI).